Forwards
Hannah Botterman (Bristol Bears), Sarah Bern (Bristol Bears), Mackenzie Carson (Gloucester-Hartpury), Kelsey Clifford (Saracens), Lizzie Hanlon (Harlequins), Maud Muir (Gloucester-Hartpury), Lark Atkin-Davies (Bristol Bears), Amy Cokayne (Leicester Tigers), Connie Powell (Harlequins), Zoe Aldcroft (Gloucester-Hartpury), Sarah Beckett (Gloucester-Hartpury), Rosie Galligan (Saracens), Lilli Ives Campion (Loughborough Lightning), Abbie Ward (Bristol Bears), Georgia Brock (Gloucester-Hartpury), Abi Burton (Trailfinders Women), Maddie Feaunati (Exeter Chiefs), Sadia Kabeya (Loughborough Lightning), Alex Matthews (Gloucester-Hartpury), Marlie Packer (Saracens), Morwenna Talling (Sale Sharks).
Backs
Natasha Hunt (Gloucester-Hartpury), Lucy Packer (Harlequins), Flo Robinson (Exeter Chiefs), Holly Aitchison (Bristol Bears), Zoe Harrison (Saracens), Fran Goldthorp (Loughborough Lightning), Tatyana Heard (Gloucester-Hartpury), Megan Jones (Leicester Tigers), Helena Rowland (Loughborough Lightning), Emily Scarratt (Loughborough Lightning, 116 caps) Reneeqa Bonner (Bristol Bears), Jess Breach (Saracens), Abby Dow (Trailfinders Women), Claudia MacDonald (Exeter Chiefs), Mia Venner (Gloucester-Hartpury), Ellie Kildunne (Harlequins), Emma Sing (Gloucester-Hartpury).